Year: 2018 | Month: June | Volume 5 | Issue 1
Isolation and Characterization of Plant Growth Promoting Arsenic-resistant Bacteria and Possible Application in Bioremediation in West Bengal
Aritri Laha1
Somnath Bhattacharyya1
Gouranga Sundar Mandal2*
Sanjoy Guha Roy3 and Sukanta Pal2
DOI:10.30954/2347-9655.01.2018.6
Abstract:
Soil arsenic contamination is a widespread problem in many developing countries including Bangladesh and India. In recent years development of modern innovative technologies for the removal of arsenic from soil has become an interesting topic for research. In this present study, ten bacteria were isolated besed on their arsenic resistant capacity. Among of them two rod shaped Gram-negative bacteria are being reported, isolated from arsenic affected soil of Chakdaha block of Nadia, West Bengal, India, which can tolerate arsenate concentration up to 409mM and 46mM of arsenite concentration. From biochemical analysis and 16S rRNA sequencing, they were identified as Burkholderia cepacia and Burkholderia metallica respectively. The isolates AL-1 and AL73 can remove 29% of arsenite and 30% and 29% of arsenate, respectively from arsenic containing culture media. Both of the isolate can oxidize arsenite to less toxic arsenate. These two also possessed plant growth-promoting traits,including phosphate-solubilization, nodule formation and IAA-like molecules. In addition, the ability of As-resistant isolates to grow over wide ranges of pH and temperatures signify their potential application for sustainable bioremediation of As in the environment.
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